Detection of Small Numbers of Campylobacter jejuni and Campylobacter coli Cells in Environmental Water, Sewage, and Food Samples by a Seminested PCR Assay
نویسندگان
چکیده
منابع مشابه
PCR-ELISAs for the detection of Campylobacter jejuni and Campylobacter coli in poultry samples.
Campylobacter species, primarily Campylobacter jejuni and Campylobacter coli, are regarded as a major cause of human gastrointestinal disease, commonly acquired by eating undercooked chicken. We describe a PCR-ELISA for the detection of Campylobacter species and the discrimination of C. jejuni and C. coli in poultry samples. The PCR assay targets the 16S/23S ribosomal RNA intergenic spacer regi...
متن کاملPrevalence of Campylobacter jejuni and Campylobacter coli in captive wildlife species of India
Campylobacteriosis is an important zoonotic disease and the prevalence of Campylobacter is largely unknown in the wildlife of India. A total of 370 samples, comprising of 314 fresh faecal samples from apparently healthy captive wild animals and birds, 30 stool swabs from animal care takers and 26 samples of the animals’ food and water were collected from G. B. Pant High Altitude Zoo, Nainital, ...
متن کاملidentification of campylobacter jejuni and campylobacter coli from diarrheic samples using pcr
background: campylobacter jejune and c. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. in this study identification of campylobacter jejuni and campylobacter coli from samples using pcr was explored. methods: detection was performed using diarrheal samples collected 8 from 117 children. the genomic dna of samples was extracted by phenol-chloroform me...
متن کاملDetection of cytolethal distending toxin (cdt) genes of Campylobacter Jejuni and Coli in fecal samples of pet birds in Iran
BACKGROUND: Campylobacteriosis is one of the most commonbacterial intestinal disorders of humans in many countries.A wide range of domestic and wild bird species have been identifiedas natural reservoirs. OBJECTIVES: The aim of this studywas to determine the Campylobacter spp from pet birds andscreen the determined Campylobacters for presence of virulencecytolethal distending toxin (cdt) genes....
متن کاملA Molecular Survey of Campylobacter jejuni and Campylobacter Coli Virulence and Diversity
Background: The aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis of Campylobacter spp. isolated from children with diarrhea in Iran. Methods: A total of 200 stool specimens were obtained from children under 5 years during July 2012 to July 2013. Detection of C. jejuni and C. coli was perfo...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.4.1636-1643.1999